Manuscripts
Preprints
Atlas of Tsc Segas Holmberg, Jennie C. and Shankar, Vijay and Lyman, Rachel and Mackay, Trudy FC and Feliciano, David M. (Cell Press) iScience Under Review

A Bitopic mTORC Inhibitor Reverses Neural Phenotypes in a Tuberous Sclerosis Complex Murine Model of SEGA-like Hamartomas Mukherjee S, Wolan MJ, Scott MK, Riley VA, Feliciano DM  
MS ID#: BIORXIV/2025/636465


Published

Atlas of Tsc Segas Holmberg, Jennie C. and Shankar, Vijay and Lyman, Rachel and Mackay, Trudy FC and Feliciano, David M. , Sneak Peek, SSRN: https://ssrn.com/abstract=5204276 or http://dx.doi.org/10.2139/ssrn.5204276

April 08, 2025

TSC-mTORC1 Pathway in Postnatal V-SVZ Feliciano DM* and Bordey A Cellular and Molecular Biology of Neurodevelopment Biomolecules Accepted April 09, 2025 https://www.mdpi.com/2218-273X/15/4/573 https://www.preprints.org/manuscript/202503.1103

Protocol for Electroporating and Isolating Murine (Sub-) Ventricular Zone Cells for Single Nuclei Omics Jennie C Holmberg; Victoria A Riley; Aidan M Sokolov; Sulagna Mukherjee; David Matthew Feliciano Star Protocols (Cell Press) May 8, 2024
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Tsc2 coordinates neuroprogenitor differentiation. VA Riley, V Shankar, JC Holmberg, AM Sokolov, VN Neckles, K Williams, R Lyman, TFC Mackay,  DM Feliciano iScience (Cell Press) 9 November 2023 https://doi.org/10.1016/j.isci.2023.108442
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Modeling Genetic Mosaicism of the Mammalian Target of Rapamycin Pathway in the Cerebral Cortex    
Feliciano, D.M.     Front. Mamm. Sci. Sec. Nervous System and Cognate Behaviors        26-Jan    2023    Frontiers Media SA

Do Neural Stem Cell Extracellular Vesicles Help EVade Depression?    
Feliciano, D.M.     Neuroscience            1    2023 Elsevier

Tsc2 shapes olfactory bulb granule cell molecular and morphological characteristics    Riley, Victoria; Holmberg, Jennie C; Sokolov, Aidan M.; Feliciano, David M.;     Frontiers in Molecular Neuroscience            20-Jan    2022   Frontiers

Slc7a5 regulation of neural development    Sokolov, Aidan M; Feliciano, David M;     Neural Regeneration Research    16 10    1994    2021    Wolters Kluwer--Medknow Publications

Slc7a5 神经发育调控    Sokolov, Aidan M; Feliciano, David M;     中国神经再生研究 (英文版)    16    10    1994    2021    

From seed to flower: blossoming of microglia in development and brain repair    Neckles, Victoria N; and Feliciano, David M;     Cell and Tissue Research                2021    Springer Nature

A Subependymal Giant Cell Astrocytoma (SEGA) Mouse Model    Feliciano, David M; Clemson University;                     2021    

The neurodevelopmental pathogenesis of tuberous sclerosis complex (TSC)    Feliciano, David M;     Frontiers in Neuroanatomy    14        39    2020    Frontiers Media SA

The amino acid transporter Slc7a5 regulates the mTOR pathway and is required for granule cell development    Sokolov, Aidan M; Holmberg, Jennie C; Feliciano, David M;     Human Molecular Genetics    29    18    3003-3013    2020    Oxford University Press

Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells    Morton, Mary C; Neckles, Victoria N; Feliciano, David M;     Stem Cell Niche: Methods and Protocols            75-85    2019    Springer New York

A transgenic inducible GFP extracellular-vesicle reporter (TIGER) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesicles    Neckles, Victoria N; Morton, Mary C; Holmberg, Jennie C; Sokolov, Aidan M; Nottoli, Timothy; Liu, Don; Feliciano, David M;     Scientific Reports    9    1    3094    2019    Nature Publishing Group UK London

TSC1 Gene Therapy AAVerts Neurological Associated Mortality    Feliciano, David M;     Molecular Therapy                2019    Cell Press

Dendrite growth and the effect of ectopic Rheb expression on cortical neurons    Sokolov, Aidan M; Seluzicki, Caitlin M; Morton, Mary C; Feliciano, David M;     Neuroscience letters    671        140-147    2018    Elsevier

Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial Morphogen    Morton, Mary; Neckles, Victoria; Seluzicki, Caitlin; Feliciano, David;     Cell Reports                2018    Cell

Neonatal subventricular zone neural stem cells release extracellular vesicles that act as a microglial morphogen    Morton, Mary C; Neckles, Victoria N; Seluzicki, Caitlin M; Holmberg, Jennie C; Feliciano, David M;     Cell reports    23    1    78-89    2018    Elsevier

Hypoxia-inducible factor-1a contributes to dendritic overgrowth in tuberous sclerosis    Zhang, Longbo; Feliciano, David M; Huang, Tianxiang; Zhang, Shiliang; Bordey, Angélique;     Neuroscience letters    612        43-47    2016    Elsevier

Switching on mTORC1 induces neurogenesis but not proliferation in neural stem cells of young mice    Mahoney, Colleen; Feliciano, David M; Bordey, Angélique; Hartman, Nathaniel W;     Neuroscience Letters    614        112-118    2016    Elsevier

Neurovesicles in brain development    Morton, Mary C; Feliciano, David M;     Cellular and molecular neurobiology    36        409-416    2016    Springer US

Noncanonical sites of adult neurogenesis in the mammalian brain    Feliciano, David M; Bordey, Angélique; Bonfanti, Luca;     Cold Spring Harbor perspectives in biology    7    10    a018846    2015    Cold Spring Harbor Lab

Embryonic cerebrospinal fluid nanovesicles carry evolutionarily conserved molecules and promote neural stem cell amplification    Feliciano, David M; Zhang, Shiliang; Nasrallah, Carole M; Lisgo, Steven N; Bordey, Angélique;     PloS one    9    2    e88810    2014    Public Library of Science

MEK-ERK1/2-dependent FLNA overexpression promotes abnormal dendritic patterning in tuberous sclerosis independent of mTOR    Zhang, Longbo; Bartley, Christopher M; Gong, Xuan; Hsieh, Lawrence S; Lin, Tiffany V; Feliciano, David M; Bordey, Angélique;     Neuron    84    1    78-91    2014    Elsevier

Cerebrospinal fluid extracellular vesicles undergo age dependent declines and contain known and novel non-coding RNAs    Tietje, Ashlee; Maron, Kourtney N; Wei, Yanzhang; Feliciano, David M;     PloS one    9    11    e113116    2014    Public Library of Science

Newborn cortical neurons: only for neonates?    Feliciano, David M; Bordey, Angélique;     Trends in neurosciences    36    1    51-61    2013    Elsevier

Hypoxia-inducible factor 1a is a Tsc1-regulated survival factor in newborn neurons in tuberous sclerosis complex    Feliciano, David M; Zhang, Shiliang; Quon, Jennifer L; Bordey, Angélique;     Human molecular genetics    22    9    1725-1734    2013    Oxford University Press

Neonatal subventricular zone electroporation    Feliciano, David M; Lafourcade, Carlos A; Bordey, Angélique;     JoVE (Journal of Visualized Experiments)        72    e50197    2013    

A circuitry and biochemical basis for tuberous sclerosis symptoms: from epilepsy to neurocognitive deficits    Feliciano, David M; Lin, Tiffany V; Hartman, Nathaniel W; Bartley, Christopher M; Kubera, Cathryn; Hsieh, Lawrence; Lafourcade, Carlos; O’Keefe, Rachel A; Bordey, Angelique;     International Journal of Developmental Neuroscience    31    7    667-678    2013    No longer published by Elsevier

Rheb activation in subventricular zone progenitors leads to heterotopia, ectopic neuronal differentiation, and rapamycin-sensitive olfactory micronodules and dendrite hypertrophy of newborn neurons    Lafourcade, Carlos A; Lin, Tiffany V; Feliciano, David M; Zhang, Longbo; Hsieh, Lawrence S; Bordey, Angélique;     Journal of Neuroscience    33    6    2419-2431    2013    Society for Neuroscience

mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase 1/2, to regulate neural stem cell self-renewal in vivo    Hartman, Nathaniel W; Lin, Tiffany V; Zhang, Longbo; Paquelet, Grace E; Feliciano, David M; Bordey, Angélique;     Cell reports    5    2    433-444    2013    Elsevier

Postnatal neurogenesis generates heterotopias, olfactory micronodules and cortical infiltration following single-cell Tsc1 deletion    Feliciano, David M; Quon, Jennifer L; Su, Tiffany; Taylor, M Morgan; Bordey, Angélique;     Human molecular genetics    21    4    799-810    2012    Oxford University Press

A regulatory feedback loop between Ca2+/calmodulin-dependent protein kinase kinase 2 (CaMKK2) and the androgen receptor in prostate cancer progression    Karacosta, Loukia G; Foster, Barbara A; Azabdaftari, Gissou; Feliciano, David M; Edelman, Arthur M;     Journal of Biological Chemistry    287    29    24832-24843    2012    Elsevier

Abstract A10: CaMKK2 regulates cellular proliferation and androgen receptor activity during prostate cancer progression    Karacosta, Loukia G; Foster, Barbara A; Gissou, Azabdaftari; Feliciano, David M; Edelman, Arthur M;     Cancer Research    72    4_Supplement    A10-A10    2012    American Association for Cancer Research

CaMKK2 regulates cellular proliferation and androgen receptor activity during prostate cancer progression    Karacosta, Loukia Georgiou; Foster, Barbara A; Azabdaftari, Gissou; Feliciano, David M; Edelman, Arthur M;                     2012    Federation of American Societies for Experimental Biology

Single-cell Tsc1 knockout during corticogenesis generates tuber-like lesions and reduces seizure threshold in mice    Feliciano, David M; Su, Tiffany; Lopez, Jean; Platel, Jean-Claude; Bordey, Angélique;     The Journal of clinical investigation    121    4    1596-1607    2011    American Society for Clinical Investigation

Un modèle murin de sclérose tubéreuse de Bourneville pour comprendre le processus d’épilepsie associé aux tubers corticaux    Feliciano, David; Bordey, Angélique;     médecine/sciences    27    3    328-330    2011    Éditions EDK, Groupe EDP Sciences

Mouse model of tuberous sclerosis complex    Feliciano, David; Bordey, Angelique;     Medecine Sciences: M/S    27    3    328-330    2011    

Repression of Ca2+/calmodulin-dependent protein kinase IV signaling accelerates retinoic acid-induced differentiation of human neuroblastoma cells    Feliciano, David M; Edelman, Arthur M;     Journal of Biological Chemistry    284    39    26466-26481    2009    Elsevier

Signaling via CaMKIV in human BE (2) C neuroblastoma cells    Feliciano, David Matthew;                     2008    State University of New York at Buffalo

CaMKIV Regulates Survival/Proliferation of Neuroblastoma Cells    Feliciano, David Matthew; Edelman, Arthur M;                     2008    Federation of American Societies for Experimental Biology
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INVITED LECTURES
Feliciano DM Harvard Medical School, Boston Children’s Hospital, Department of Pathology, How the Neonatal Subventricular Zone Relates to Neurodevelopmental Disorders, September 23, 2022
 
Feliciano DM TSC-Associated Neuropsychiatric Disorders (TAND) international minisymposium, Tsc2 Shapes Molecular and Cellular Characteristics of Granule Cells, April 21, 2022
 
Feliciano DM TSC-Associated Neuropsychiatric Disorders (TAND) international minisymposium, Tsc2 Shapes Molecular and Cellular Characteristics of Granule Cells, April 21, 2022
 
Feliciano DM A Tor of the Developing Brain South Carolina Autism and Neurodevelopmental Disorders (SCAND) Research Symposium March 05, 2021
 
Feliciano DM C.S. Mott Center for Human Growth and Development, NIH Pregnancy and Perinatology Branch (PPB of NICHD), March 19-20, 2019
 
Feliciano DM Cold Spring Harbor Asia, “Biology of Extracellular Vesicles”, Suzhou, China November 12-16, 2018
 
Feliciano DM University of South Carolina, Department of Biology, “A Neural Stem Cell-Extracellular Vesicle-Microglia Axis”, September 2018
 
Feliciano DM University of Georgia, Neuroscience Program, “A Neural Stem Cell-Extracellular Vesicle-Microglia Axis”, October 2018
 
Feliciano DM Michigan State University (MSU), Department of Pediatrics, College Human Medicine, Invited Talk titled “Identifying Mechanisms and Therapeutic Approaches for Tuberous Sclerosis Complex and mTOR-Dependent Neurodevelopmental Disorders” June 26, 2017
 
Feliciano DM, Morton MC, Neckles VN, Seluzicki CM, Neonatal subventricular zone neural stem cells release extracellular vesicles that function as a non-canonical microglial morphogen. Society for Neuroscience Postnatal Neurogenesis and Stem Cell Functions November 15, 2017 Invited Nanosymposium Oral Presentation
 
Feliciano DM, Neurovesicles in Brain Development Southeastern Regional Developmental Biology Meeting May 19, 2017
 
Feliciano DM, A neurodevelopmental regulator of the mtor pathway Society for Neuroscience Annual Meeting October 2015 Invited Nanosymposium Oral Presentation
 
Feliciano DM, A neurodevelopmental regulator of the mtor pathway Society for Developmental Biology Southeast Regional Meeting, May 2015, Oral Presentation
 
Feliciano DM University of Georgia at Athens (UGA), Department of Physiology and Pharmacology, mTOR Regulation and Cortical Development October 2016
Feliciano DM Allen Brain Institute: Evolution of Cerebrocortical Development, 2013
 
Feliciano DM Saint Bonaventure University: Evolution of Cerebrocortical Development, 2013
Feliciano DM University of New England: Pharmacologically Resistant Epilepsy: Lessons Learned from Tuberous Sclerosis Complex, 2013
 
Feliciano DM Des Moines University: Molecular Mechanisms Regulating Fetal Brain Development and What we can Learn from Tuberous Sclerosis Complex, 2013
 
Feliciano DM Tuberous Sclerosis Complex: Mouse Models and Mechanisms. 2012 Yale Department of Physiology
 
Feliciano DM Single-cell knockout of Tsc1 in utero generates cortical tuber-like lesions and heterotopic nodules with cytomegalic neurons. 2011 Yale Club Neurobiology
 
Feliciano DM, Single-cell knockout of Tsc1 in utero generates cortical tuber-like lesions and heterotopic nodules with cytomegalic neurons. 2010 Society for Neuroscience Selected for Mini-symposium-Animal models of developmental disorders. Invited Oral Presentation
 
Feliciano DM, Combining old and new tools to model malformations of cortical development, What’s Next: A Young Investigator Workshop. Invited Speaker at American Epilepsy Society 2011 Invited Oral Presentation
 
Conference Poster Presentations
 
Posters:
 
International TSC Alliance, Tsc2 Regulates Differentiation. September 26, 2023 Riley VA, Shankar V, Holmberg JC, Sokolov AM, Neckles VN, Williams K, Lyman R, Mackay TFC, Feliciano DM
 
Society for Neuroscience, Tsc2 Shapes Molecular and Cellular Characteristics of Granule Cells, November 12-16, 2022, Jennie C. Holmberg, Victoria A. Riley, Aidan M. Sokolov, David M. Feliciano
 
Society for Neuroscience, Inactivating mutations in Tsc2 in inhibitory interneuron neuroprogenitors of the V-SVZ result in somatic mosaicism and the formation of SEGA-like hamartomas and striatal nodules, November 12-16, 2022, Victoria A. Riley, Jennie C. Holmberg, Aidan M. Sokolov, David M. Feliciano
 
Southeast Regional Society for Developmental Biology Conference 2022, June 01, 2022 -June 03, 2022, Determination of the effect of Tsc2 deletion on neurogenesis in the hippocampus, Melanie Garcia, Jennie C. Holmberg, Victoria A. Riley, David M. Feliciano
 
Southeast Regional Society for Developmental Biology Conference 2022, June 01, 2022 -June 03, 2022, Loss of Tsc2 in Late Inhibitory Neural Progenitors Causes Brain Lesion, Victoria A. Riley, Jennie C. Holmberg, Aidan M. Sokolov, David M. Feliciano
 
COBRE in Human Genetics Summer Symposium 2022, Tsc2 Shapes Molecular and Cellular Characteristics of Granule Cells, April 21, 2022, Jennie C. Holmberg, Victoria A. Riley, Aidan M. Sokolov, David M. Feliciano
 
COBRE in Human Genetics Summer Symposium 2022, April 21, 2022, Loss of Tsc2 in late inhibitory neural progenitors causes brain lesions Victoria A. Riley, Jennie C. Holmberg, Aidan M. Sokolov, David M. Feliciano
 
COBRE in Human Genetics Summer Symposium 2022, Determination of the Effect of TSC2 Deletion on Neurogenesis in the Hippocampus, April 21, 2022, Melanie Garcia, Jennie C. Holmberg, Victoria A. Riley, David M. Feliciano
 
COBRE in Human Genetics Summer Symposium 2022, Characterization of Microglia in Development and Disease, April 21, 2022, Victoria Neckles, Jennie C. Holmberg Victoria A. Riley, Aidan M. Sokolov, David M. Feliciano
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International TSC Research Conference 2021 (ITSCRC2021), A Mouse Model of Subependymal Giant Cell Astrocytomas, 17-19 June 2021, 2022, Victoria A. Riley, Aidan M. Sokolov, Jennie C. Holmberg, David M. Feliciano
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TSC-Associated Neuropsychiatric Disorders (TAND) international minisymposium, Loss of Tsc2 in late inhibitory neural progenitors causes brain lesions, April 21, 2022, Victoria A. Riley
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COBRE in Human Genetics Summer Symposium 2022, Tsc2 Shapes Molecular and Cellular Characteristics of Granule Cells, April 21, 2022, Jennie C. Holmberg Victoria A. Riley, Aidan M. Sokolov, David M. Feliciano
 
COBRE in Human Genetics Summer Symposium 2022, Determination of the Effect of TSC2 Deletion on Neurogenesis in the Hippocampus, April 21, 2022, Melanie Garcia, Jennie C. Holmberg, Victoria A. Riley, Aidan M. Sokolov, David M. Feliciano
 
COBRE in Human Genetics Summer Symposium 2022, Characterization of Microglia in Development and Disease, April 21, 2022, Victoria Neckles, Jennie C. Holmberg Victoria A. Riley, Aidan M. Sokolov, David M. Feliciano
Seluzicki, C.M., Holmberg, J.C., Morton M.C., Feliciano DM Slc7a5 Regulates Newborn Cortical Pyramidal Neuron mTOR Pathway Activity and Dendritogenesis March 2016 Neurogenesis (Organized by and contributions to the CSHL Press Monograph, Neurogenesis)
 
Feliciano DM, Morton MC, Neckles VN, Seluzicki CM, Neonatal subventricular zone neural stem cells release extracellular vesicles that function as a non-canonical microglial morphogen. National Institutes of Health Extracellular RNA Communication Consortium 9th Investigators’ Meeting November 6-7, 2017
 
Sokolov AM and Feliciano DM, mTOR-dependent ULK1 phosphorylation is rapamycin resistant in neural derived cells. Society for Neuroscience Annual Meeting Neurodevelopmental Disorders: Molecular and Cellular Mechanisms I Poster Session November 14, 2017
 
Feliciano DM, Morton MC, Seluzicki CM, Holmberg, JC, A neurodevelopmental source of extracellular vesicles. Society for Neuroscience November 13, 2016.
 
Morton MC, Holmberg JC, Cuffe SN, Kokolas A, Feliciano DM Inhibition of Neutral Sphingomeylinases Disrupts Embryonic Cortical Neurogenesis Society for Developmental Biology Southeast Regional Meeting, May 2015, Presentation
 
Tietje A, Maron K, Bordey A, Wei Y, Feliciano DM Temporal dynamics of cerebrospinal fluid vesicles. Society for Neuroscience Annual Meeting November 2014
 
Feliciano DM, Quon JL, Bordey A Hif1a is a Tsc1 regulated factor required for newborn neuron survival, Cold Spring Harbor Laboratories, PTEN Meeting March 2012
 
Feliciano DM, Taylor MM, Su T, Quon JL, Bordey A Focal Postnatal Tsc1 recombination in the Subventricular Zone induces mTOR pathway activation, reduces neuroblast migration rates, and generates ectopically localized neurons Drug Discovery Summit, Tuberous Sclerosis Alliance 2011
 
Feliciano DM, Su T, Bordey A Single-cell knockout of Tsc1 in utero generates cortical tuber-like lesions and heterotopic nodules with cytomegalic neurons American Epilepsy Society 64th Annual Meeting 2010
 
Feliciano DM, Su T, Bordey A Biallelic inactivation of TSC1 in radial Glia results in mTOR pathway activation Cold Spring Harbor Laboratories PTEN Meeting March 2010
 
Feliciano DM, Su T, Platel JC, Bordey A Embryonic Single Cell Knockout of TSC1 recapitulates the phospho- S6 Giant Cell Phenotype of Tuberous Sclerosis Complex, Tuberous Sclerosis Alliance Meeting, October 2009
 
Feliciano DM and Edelman AM CaMKIV Regulates Proliferation/Survival of Neuroblastoma Cells April 2008, ASPET Centennial Meeting